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The HUGO Journal

Official Journal of the Human Genome Organisation

Fig. 6 | The HUGO Journal

Fig. 6

From: Genome-wide identification and annotation of HIF-1α binding sites in two cell lines using massively parallel sequencing

Fig. 6

Profiles of RNA polymerase II binding and histone modification in the regions surrounding HIF-1α binding sites. Average tag concentrations (y-axis) obtained in the ChIP-Seq analyses of pol II (a, e), H3K4me3 (b, f), H3Ac (c, g) and H3K27me3 (d, h) are plotted for each genomic coordinate (x-axis). The observed profiles are shown for DLD-1 (a–d) and TIG-3 cells (e–h). In each panel, red and blue lines represent the tag counts under hypoxia and normoxia for the IP samples, respectively. Green and sky blue lines represent the results obtained for the background control (whole cell extract samples). The putative binding site of HIF-1α, represented by HRE, was defined as position 0 (x-axis). For each panel, the tag counts for 290 and 221 binding sites, which have HRE among the HIF-1α target TSCs (either geneic or intergenic TSCs), were averaged in DLD-1 and TIG-3 cells, respectively. (Color figure online)

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