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Official Journal of the Human Genome Organisation

Table 1 Properties of the p53 tetramerization domain mutants

From: Parallel analysis of tetramerization domain mutants of the human p53 protein using PCR colonies

Mutant

WT AA

Mutant AA

Polony μ (h−1)

Red/White

%WT activity

p53 Western blot

Leu330Ala

 

Hydrophobic uncharged

3.7 ± 0.6

5.6 ± 4.2

Leu330Arg

Hydrophilic basic

4.3 ± 0.6

Leu330Asn

Hydrophilic uncharged

4.3 ± 0.6

7.2 ± 14.4

Leu330Asp

Hydrophilic acidic

5.0 ± 0

47.6 ± 8.0

121.7% ± 8.3%

Leu330Cys

Uncharged hydrophobic

0.174

1.7 ± 0.6

Leu330Gln

Hydrophilic uncharged

3.0 ± 0

Leu330Glu

Hydrophilic acidic

5.0 ± 0

54.4 ± 22.3

Leu330Gly

Uncharged

5.0 ± 0

5.5 ± 5.4

Leu330His

Hydrophilic basic

4.3 ± 0.6

7.6 ± 10.0

Leu330Ile

Hydrophobic uncharged

0.196

1.3 ± 0.6

250.5 ± 7.4

Leu330Lys

Hydrophilic basic

5.0 ± 0

29.9 ± 16.2

Leu330Met

Hydrophobic uncharged

0.205

2.0 ± 1.0

72.8 ± 4.5

Leu330Phe

Hydrophobic uncharged

0.192

1.0 ± 0

88.9% ± 11.3%

Leu330Pro

Uncharged

4.0 ± 0

Leu330Ser

Hydrophilic uncharged

0.186

4.7 ± 0.6

Leu330Thr

Hydrophilic uncharged

0.193

4.3 ± 0.6

Leu330Trp

Hydrophobic uncharged

4.0 ± 0

98.4% ± 7.3%

Leu330Tyr

Hydrophobic ionizable

0.198

1.3 ± 0.6

150.6 ± 27.9

100.0%

Leu330Val

Hydrophobic uncharged

Hydrophobic uncharged

0.195

1.3 ± 0.6

164.9 ± 22.6

Gln331Ala

 

Hydrophobic uncharged

0.205

1.0 ± 0

77.6 ± 8.0

Gln331Arg

Hydrophilic basic

0.202

1.7 ± 0.6

Gln331Asn

Hydrophilic uncharged

0.203

2.0 ± 0

80.5% ± 17.1%

Gln331Asp

Hydrophilic acidic

0.211

1.3 ± 0.6

Gln331Cys

Uncharged hydrophobic

3.0 ± 0

Gln331Glu

Hydrophilic uncharged

0.203

2.0 ± 0

Gln331Gly

Hydrophilic acidic

0.203

1.7 ± 0.6

Gln331His

Uncharged

0.217

1.0 ± 0

153.0

Gln331Ile

Hydrophilic basic

0.204

2.0 ± 0

Gln331Leu

Hydrophobic uncharged

0.209

2.0 ± 0

Gln331Lys

Hydrophilic basic

0.199

1.0 ± 0

Gln331Met

Hydrophobic uncharged

0.203

1.3 ± 0.6

Gln331Phe

Hydrophobic uncharged

0.186

2.0 ± 0

46.3

Gln331Pro

Uncharged

0.197

3.3 ± 0.6

37.9 ± 13.2

Gln331Ser

Hydrophilic uncharged

0.184

1.0 ± 0

Gln331Thr

Hydrophilic uncharged

0.194

2.0 ± 0

56.2 ± 6.8

Gln331Trp

Hydrophobic uncharged

0.192

4.0 ± 0

107.7 ± 14.7

Gln331Tyr

Hydrophobic ionizable

2.7 ± 0.6

Gln331Val

Hydrophilic uncharged

Hydrophobic uncharged

2.3 ± 0.6

Ile332 Ala

 

Hydrophobic uncharged

4.3 ± 0.6

14.7

Ile332Arg

Hydrophilic basic

5.0 ± 0

Ile332Asn

Hydrophilic uncharged

0.205

5.0 ± 0

33.1

Ile332Asp

Hydrophilic acidic

5.0 ± 0

Ile332Cys

Uncharged hydrophobic

0.202

1.0 ± 0

86.1% ± 6.9%

Ile332Gln

Hydrophilic uncharged

5.0 ± 0

Ile332Glu

Hydrophilic acidic

5.0 ± 0

26.9 ± 6.9

122.2% ± 25.5%

Ile332Gly

Uncharged

4.0 ± 0

Ile332His

Hydrophilic basic

5.0 ± 0

110.9% ± 12.0%

Ile332Leu

Hydrophobic uncharged

0.198

1.0 ± 0

84.2

Ile332Lys

Hydrophilic basic

5.0 ± 0

Ile332Met

Hydrophobic uncharged

0.190

2.0 ± 0

179.2 ± 35.8

Ile332 Phe

Hydrophobic uncharged

5.0 ± 0

Ile332Pro

Uncharged

5.0 ± 0

Ile332Ser

Hydrophilic uncharged

4.7 ± 0.6

35.4

Ile332Thr

Hydrophilic uncharged

0.192

2.3 ± 0.6

101.0% ± 6.6%

Ile332Trp

Hydrophobic uncharged

4.7 ± 0.6

Ile332Tyr

Hydrophobic ionizable

4.7 ± 0.6

Ile332Val

Hydrophobic uncharged

Hydrophobic uncharged

0.198

2.7 ± 0.6

56.0