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The HUGO Journal

Official Journal of the Human Genome Organisation

Fig. 4 | Genomic Medicine

Fig. 4

From: 5α-androstane-3α,17β-diol selectively activates the canonical PI3K/AKT pathway: a bioinformatics-based evidence for androgen-activated cytoplasmic signaling

Fig. 4

Western blot analysis of 3α-diol-regulated AKT expression and phosphorylation in LNCaP cells. (a) Temporal regulation of AKT expression and phosphorylation between 15 min and 8 h in LNCaP cells treated with 10−8 M 3α-diol. (b) Temporal regulation of AKT expression and phosphorylation in AR-silenced LNCaP cells. LNCaP cells were transiently transfected with the pSiAR-EGFP plasmid, and subjected to serum deprivation followed by 10−8 M 3α-diol stimulation. Levels of AKT expression and phosphorylation were determined between 30 min and 8 h following 3α-diol stimulation. Experiments were repeated twice and a representative result was presented

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