Table 1 List of final human non-homolog targets with corresponding interproscan annotation, functional significance, and host-pathogen interactions

NP_345734.1

Alcohol dehydrogenase, Zinc-containing

S. pneumoniae

Not required

Modulation of ZN+

Maintenance of the intracellular homeostasis of Zn+ ions is important for virulence and host interaction in S. pneumoniae (Kloosterman et al. 2007).

NP_345735.1

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

S. pneumoniae

Not required

Isoprenoid biosynthesis, essential for cell viability and virulence

Isoprenoids are localized in membrane of S. pneumoniae and membrane components and essential for exhibiting virulence in host (Wilding et al. 2000)

NP_438218.1

Hypothetical protein HI0045

H. influenzae

Conserved hypothetical protein, YTFJ

Un- characterized conserved protein

Uncharacterized conserved protein

NP_439769.1

Hypothetical protein HI1627

H. influenzae

Endoribonuclease/chorism_mut-like, YJGF/chorismate_mutase-like

Modulation of fimbria expression genes, PUR and ILE, inhibit translation and even serve as mammalian tumor associated ANTIGEN

The airway colonization of H. influenzae in host is facilitated by fimbria (Read et al. 1992; Van Alphen and van Ham 1994)

NP_439869.1

Hypothetical protein HI1728

H. influenzae

Natural resistance-associated macrophage protein, function: transport, component: membrane

Metal ion transporters

Metal ion transport plays a key role in pathogenesis in the host (Agranoff and Krishna 1998)

NP_439212.1

Hypothetical protein HI1053

H. influenzae

Carboxymuconol-actone decarboxylase

Aromatic compound degradation

Unknown

NP_439576.1

Hypothetical protein HI1427

H. influenzae

Unintegrated, putative periplasmic-binding protein CBIK

Periplasmic intermediate

Periplasmic proteins mediate non-pilus adhesins attachment to human epithelial cells, an essential step during colonization of H. influenzae (St Geme and Grass 1998)

NP_439140.1

Cell filamentation protein

H. influenzae

Not required

Cell division and chromosome partitioning

Filamentation proteins also play an important role in non-beta lactamase mediated beta-lactam resistance in H. influenzae despite serotype, origin of isolation, or geographic distribution (Clairoux et al. 1992)