Official Journal of the Human Genome Organisation
From: Review of massively parallel DNA sequencing technologies
Method | Advantages | Disadvantages |
---|---|---|
PCR | High sensitivity, specificity, reproducibility and uniformity | High cost, low throughput, and cannot be used for large regions or a very large number of genes |
Circularisation | Low cost (if many samples), easy to use, high sensitivity and specificity | Uniformity and sensitivity depends on design of probes. Cannot be used for a very large number of genes |
Hybrid capture | Medium cost, easy to use, high sensitivity and specificity. Can target large sections of DNA and large numbers of genes | Uniformity and sensitivity depends on design of probes. Array design may be rather inflexible |