Fig. 3
From: Evidence of EGR1 as a differentially expressed gene among proliferative skin diseases
Egr-1 limits epidermal cell hyperproliferation. (a) Proliferative output of sub-confluent epidermal cells in growth media. The effect of wild-type Egr-1, the Egr-1 DNA binding domain deletion mutant (DBD) compared to marker gene control is shown in normal human keratinocytes (NL), in keratinocytes immortalized with HPV 18 E6/E7 and in malignant SCC-25 cells. Total cell numbers 6 days after transduction are shown from triplicate independent transductions initiated with identical cell numbers. # = P < 0.01; * = P < 0.05 for differences between Egr-1 transduced cells and control cells. (b) Reactive epidermal hyperplasia in vivo. Murine epidermis transgenic for Egr-1 (K14-Egr-1) resists PMA-induced epidermal hyperplasia; scale bar = 30 μm. (c) Quantitative comparison of epidermal cell numbers/100 μm of basement membrane zone (BMZ) in PMA treated and ethanol control Egr-1 transgenic and littermate skin (n = 4 mice per group). ** = P < 0.001 for differences between PMA treated transgenic and control skin